Artefacts:

air bubbles, cloth fibers, pollen grains, hair/fibers, glove powder


urine-23.jpg (8861 bytes)

Urine sediment, 400x

                                Cloth Fibers

Cloth fibers can often take on interesting shapes in the urine sediment. It is important not to confuse them with microorganisms. Cloth fibers, as well as dust and animal hairs, are often found in the urine sediment as contaminants.

 

 

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Urine sediment, 400x

                                Glove Powder

Glove powder is a frequent contaminant of urine sediments, especially when samples are obtained using an aseptic technique. Typically, talcum powder crystals are rounded with a small cross in their center, though their size and shape may vary slightly. These crystals should not be confused with those of clinical significance.

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Urine sediment, 400x

Hair/fibers 

This microphotograph illustrates a fiber or animal hair which is characterized by an elongated, asymmetrical, and refractile appearance. This type of artefact should not be confused with a fungal organism or parasite larva such as microfilaria. The best way to ensure that the structure in question is an artefact is to examine the slide to see if the same structure appears in other fields, shows symmetry and an absence of refractility. When the structure in question is an artefact, its appearance will generally vary.

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Urine sediment, 100x

Air Bubbles

When a coverslip is placed on the slide containing a urine sediment, small air bubbles can be trapped beneath it. These air bubbles will have a variable shape and size, and the number of bubbles will vary from field to field.   Generally, these air bubbles will remain isolated, whereas lipid droplets will tend to coalesce. The air bubbles can be seen when surrounding cells or other structures will be in focus.

 

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Urine sediment, 100x

Lipid Droplets

It is normal to find lipid droplets in the urinary sediment, especially in cats. The number and size of the lipid droplets can vary. They can be distinguished as bright rings which  float over the sediment and other structures. The droplets will be in focus when the other structures are not. Lipid droplets should not be confused with red blood cells or air bubbles.

 

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Urine sediment, 100x

 

Pollen grains

This microscopic field contains three pollen grains. These are contaminants which may be misinterpreted as parasite larvae in the oocyst stage. Compared to real oocysts, however, pollen grains will demonstrate a more variable appearance.

 

 

 

air bubbles, cloth fibers, pollen grains, hair/fibers, glove powder

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