Artefacts:
air bubbles, cloth fibers, pollen grains, hair/fibers, glove powder
Urine sediment, 400x |
Cloth fibers can often take on interesting shapes in the urine sediment. It is important not to confuse them with microorganisms. Cloth fibers, as well as dust and animal hairs, are often found in the urine sediment as contaminants.
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Urine sediment, 400x |
Glove powder is a frequent contaminant of urine sediments, especially when samples are obtained using an aseptic technique. Typically, talcum powder crystals are rounded with a small cross in their center, though their size and shape may vary slightly. These crystals should not be confused with those of clinical significance. |
Urine sediment, 400x |
This microphotograph illustrates a fiber or animal hair which is characterized by an elongated, asymmetrical, and refractile appearance. This type of artefact should not be confused with a fungal organism or parasite larva such as microfilaria. The best way to ensure that the structure in question is an artefact is to examine the slide to see if the same structure appears in other fields, shows symmetry and an absence of refractility. When the structure in question is an artefact, its appearance will generally vary. |
Urine sediment, 100x |
When a coverslip is placed on the slide containing a urine sediment, small air bubbles can be trapped beneath it. These air bubbles will have a variable shape and size, and the number of bubbles will vary from field to field. Generally, these air bubbles will remain isolated, whereas lipid droplets will tend to coalesce. The air bubbles can be seen when surrounding cells or other structures will be in focus.
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Urine sediment, 100x |
It is normal to find lipid droplets in the urinary sediment, especially in cats. The number and size of the lipid droplets can vary. They can be distinguished as bright rings which float over the sediment and other structures. The droplets will be in focus when the other structures are not. Lipid droplets should not be confused with red blood cells or air bubbles.
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Urine sediment, 100x |
This microscopic field contains three pollen grains. These are contaminants which may be misinterpreted as parasite larvae in the oocyst stage. Compared to real oocysts, however, pollen grains will demonstrate a more variable appearance.
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air bubbles, cloth fibers, pollen grains, hair/fibers, glove powder