Artefacts:
stain precipitate, pseudolarvae, glove powder
Wright's stain, 500x |
Stain precipitate is often confused with bacteria. This artefact is common in hematologic and cytologic specimens. An excess of precipitate calls for filtering the staining solution or replacing it. Precipitate can be distinguished from bacteria by its irregular and more amorphous appearance. True bacteria such as cocci will have a more discernable spherical appearance, tend to be uniform in size, and may be found in pairs or chains.
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Pseudolarvae ("pseudofilariae") Wright's stain, 500x |
("pseudo-oocyst") Wright's stain, 500x |
Textile fibers, dust, and even pollen grains can mimic parasites or larvae. Generally, their refractile appearance and lack of symmetry allows us to distinguish these from true microorganisms.
Wright's stain, 500x |
This image shows many glove (talcum) powder crystals (black arrows). These crystals are rounded with an irregular outline and often have a small "x" or t in their center. They are frequent contaminants when an aseptic sampling technique is used.
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